Ontology highlight
ABSTRACT:
SUBMITTER: Krishnakumar SS
PROVIDER: S-EPMC3668341 | biostudies-literature | 2011 Jul
REPOSITORIES: biostudies-literature
Krishnakumar Shyam S SS Radoff Daniel T DT Kümmel Daniel D Giraudo Claudio G CG Li Feng F Khandan Lavan L Baguley Stephanie Wood SW Coleman Jeff J Reinisch Karin M KM Pincet Frederic F Rothman James E JE
Nature structural & molecular biology 20110724 8
The crystal structure of complexin bound to a prefusion SNAREpin mimetic shows that the accessory helix extends away from the SNAREpin in an 'open' conformation, binding another SNAREpin and inhibiting its assembly, to clamp fusion. In contrast, the accessory helix in the postfusion complex parallels the SNARE complex in a 'closed' conformation. Here we use targeted mutations, FRET spectroscopy and a functional assay that reconstitutes Ca(2+)-triggered exocytosis to show that the conformational ...[more]