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Structure of an A-form RNA duplex obtained by degradation of 6S RNA in a crystallization droplet.

ABSTRACT: In the course of a crystallographic study of a 132 nt variant of Aquifex aeolicus 6S RNA, a crystal structure of an A-form RNA duplex containing 12 base pairs was solved at a resolution of 2.6 Å. In fact, the RNA duplex is part of the 6S RNA and was obtained by accidental but precise degradation of the 6S RNA in a crystallization droplet. 6S RNA degradation was confirmed by microscopic observation of crystals and gel electrophoresis of crystallization droplets. The RNA oligomers obtained form regular A-form duplexes containing three GoU wobble-type base pairs, one of which engages in intermolecular contacts through a ribose-zipper motif at the crystal-packing interface.

SUBMITTER: Kondo J

PROVIDER: S-EPMC3668581 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature

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Structure of an A-form RNA duplex obtained by degradation of 6S RNA in a crystallization droplet.

Kondo Jiro J Dock-Bregeon Anne Catherine AC Willkomm Dagmar K DK Hartmann Roland K RK Westhof Eric E

Acta crystallographica. Section F, Structural biology and crystallization communications 20130524 Pt 6

In the course of a crystallographic study of a 132 nt variant of Aquifex aeolicus 6S RNA, a crystal structure of an A-form RNA duplex containing 12 base pairs was solved at a resolution of 2.6 Å. In fact, the RNA duplex is part of the 6S RNA and was obtained by accidental but precise degradation of the 6S RNA in a crystallization droplet. 6S RNA degradation was confirmed by microscopic observation of crystals and gel electrophoresis of crystallization droplets. The RNA oligomers obtained form re ...[more]

PMID: 23722840

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