Using natural variation in Drosophila to discover previously unknown endoplasmic reticulum stress genes.

ABSTRACT: Natural genetic variation is a rich resource for identifying novel elements of cellular pathways such as endoplasmic reticulum (ER) stress. ER stress occurs when misfolded proteins accumulate in the ER and cells respond with the conserved unfolded protein response (UPR), which includes large-scale gene expression changes. Although ER stress can be a cause or a modifying factor of human disease, little is known of the amount of variation in the response to ER stress and the genes contributing to such variation. To study natural variation in ER stress response in a model system, we measured the survival time in response to tunicamycin-induced ER stress in flies from 114 lines from the sequenced Drosophila Genetic Reference Panel of wild-derived inbred strains. These lines showed high heterog

SUBMITTER: Chow CY 

PROVIDER: S-EPMC3670321 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature