Oscillations of sub-membrane ATP in glucose-stimulated beta cells depend on negative feedback from Ca(2+).

ABSTRACT:

Aims/hypothesis

ATP links changes in glucose metabolism to electrical activity, Ca(2+) signalling and insulin secretion in pancreatic beta cells. There is evidence that beta cell metabolism oscillates, but little is known about ATP dynamics at the plasma membrane, where regulation of ion channels and exocytosis occur.

Methods

The sub-plasma-membrane ATP concentration ([ATP]pm) was recorded in beta cells in intact mouse and human islets using total internal reflection microscopy and the fluorescent reporter Perceval.

Results

Glucose dose-dependently increased [ATP]pm with half-maximal and maximal effects at 5.2 and 9 mmol/l, respectively. Additional elevations of glucose to 11 to 20 mmol/l promoted pronounced [ATP]pm oscillations that were synchronised between neighbou

SUBMITTER: Li J 

PROVIDER: S-EPMC3671113 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature