Browse
Submit Data
Databases
API
Help

Dataset Information

5 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A C. elegans screening platform for the rapid assessment of chemical disruption of germline function.

ABSTRACT:

Background

Despite the developmental impact of chromosome segregation errors, we lack the tools to assess environmental effects on the integrity of the germline in animals.

Objectives

We developed an assay in Caenorhabditis elegans that fluorescently marks aneuploid embryos after chemical exposure.

Methods

We qualified the predictive value of the assay against chemotherapeutic agents as well as environmental compounds from the ToxCast Phase I library by comparing results from the C. elegans assay with the comprehensive mammalian in vivo end point data from the ToxRef database.

Results

The assay was highly predictive of mammalian reproductive toxicities, with a 69% maximum balanced accuracy. We confirmed the effect of select compounds on germline integrity by monitoring germline apoptosis and meiotic progression.

Conclusions

This C. elegans assay provides a comprehensive strategy for assessing environmental effects on germline function.

SUBMITTER: Allard P

PROVIDER: S-EPMC3672921 | biostudies-literature |

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Similar Datasets

A screening-based platform for the assessment of cellular respiration in Caenorhabditis elegans.

Project description: Not available

| S-EPMC5040492 | biostudies-literature

Establish a rapid and efficient chemical reprogramming platform

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-05-03 | GSE188834 | GEO

A safety screening platform for individualized cardiotoxicity assessment

Project description:Cardiotoxicity remains a major cause of drug withdrawal, partially due to lacking predictability of animal models. Additionally, risk of cardiotoxicity following treatment of cancer patients is treatment limiting. It is unclear which patients will develop heart failure following therapy. Human pluripotent stem cell (hPSC)-derived cardiomyocytes present an unlimited cell source and may offer individualized solutions to this problem. We developed a platform to predict molecular and functional aspects of cardiotoxicity. Our platform can discriminate between the different cardiotoxic mechanisms of existing and novel anthracyclines Doxorubicin (DOXO), Aclarubicin (ACLA) and Amrubicin (AMR). DOXO and ACLA unlike AMR substantially affected the transcriptome, mitochondrial membrane integrity, contractile force and transcription factor availability. Cardiomyocytes recovered fully within two or three weeks, corresponding to the intermittent clinical treatment regimen. Our system permits the study of hPSC-cardiomyocyte recovery and the effects of accumulated dose after multiple dosing, allowing individualized cardiotoxicity evaluation, which effects millions of cancer patients treated with anthracyclines annually.

2023-10-01 | GSE232331 | GEO

Establish a rapid and efficient chemical reprogramming platform (RRBS)

Project description:Chemically reprogramming somatic cells to iPSCs is time-consuming and low-efficiency. Here, we discribe a rapid chemical reprogramming condition enabling generating iPSCs from MEFs in 12 days. To further investigate the mechemisms and draw an epigenetic maps during the rapid chemical reprogramming, we performed time-course RNA-seq, ATAC-seq, RRBS and CUT&Tag (H3K4me3, H3K18la, H3K9me3, H3K27me3 and H3K27ac).

2023-05-03 | GSE188831 | GEO

Establish a rapid and efficient chemical reprogramming platform (ATAC-Seq)

2023-05-03 | GSE188824 | GEO

Establish a rapid and efficient chemical reprogramming platform (RNA-Seq)

2023-05-03 | GSE188830 | GEO

Establish a rapid and efficient chemical reprogramming platform (CUT&Tag)

2023-05-03 | GSE188826 | GEO

Establish a rapid and efficient chemical reprogramming platform [scRNA-Seq]

Project description:Chemically reprogramming somatic cells to iPSCs is time-consuming and low-efficiency. Here, we discribe a rapid chemical reprogramming condition enabling generating iPSCs from MEFs in 12 days. To further investigate the mechemisms and draw an epigenetic maps during the rapid chemical reprogramming, we performed time-course RNA-seq, ATAC-seq, RRBS, CUT&Tag (H3K4me3, H3K18la, H3K9me3, H3K27me3 and H3K27ac) and scRNA-seq.

2023-05-03 | GSE218855 | GEO

Establish a rapid and efficient chemical reprogramming platform (RNA-Seq II)

Project description:Chemically reprogramming somatic cells to iPSCs is time-consuming and low-efficiency. Here, we discribe a rapid chemical reprogramming condition enabling generating iPSCs from MEFs in 12 days. To further investigate the mechemisms and draw an epigenetic maps during the rapid chemical reprogramming, we performed time-course RNA-seq, ATAC-seq, RRBS, CUT&Tag (H3K4me3, H3K18la, H3K9me3, H3K27me3 and H3K27ac) and scRNA-seq.

2023-05-03 | GSE220554 | GEO

Assessment and Maintenance of Unigametic Germline Inheritance for C. elegans.

Project description: Not available

| S-EPMC6435406 | biostudies-literature