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A C. elegans screening platform for the rapid assessment of chemical disruption of germline function.

ABSTRACT:

Background

Despite the developmental impact of chromosome segregation errors, we lack the tools to assess environmental effects on the integrity of the germline in animals.

Objectives

We developed an assay in Caenorhabditis elegans that fluorescently marks aneuploid embryos after chemical exposure.

Methods

We qualified the predictive value of the assay against chemotherapeutic agents as well as environmental compounds from the ToxCast Phase I library by comparing results from the C. elegans assay with the comprehensive mammalian in vivo end point data from the ToxRef database.

Results

The assay was highly predictive of mammalian reproductive toxicities, with a 69% maximum balanced accuracy. We confirmed the effect of select compounds on germline integrity by monitoring germline apoptosis and meiotic progression.

Conclusions

This C. elegans assay provides a comprehensive strategy for assessing environmental effects on germline function.

SUBMITTER: Allard P

PROVIDER: S-EPMC3672921 | biostudies-literature |

REPOSITORIES: biostudies-literature

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