Unknown

Dataset Information

0

Characterization of an ntrX mutant of Neisseria gonorrhoeae reveals a response regulator that controls expression of respiratory enzymes in oxidase-positive proteobacteria.


ABSTRACT: NtrYX is a sensor-histidine kinase/response regulator two-component system that has had limited characterization in a small number of Alphaproteobacteria. Phylogenetic analysis of the response regulator NtrX showed that this two-component system is extensively distributed across the bacterial domain, and it is present in a variety of Betaproteobacteria, including the human pathogen Neisseria gonorrhoeae. Microarray analysis revealed that the expression of several components of the respiratory chain was reduced in an N. gonorrhoeae ntrX mutant compared to that in the isogenic wild-type (WT) strain 1291. These included the cytochrome c oxidase subunit (ccoP), nitrite reductase (aniA), and nitric oxide reductase (norB). Enzyme activity assays showed decreased cytochrome oxidase and nitrite reductase activities in the ntrX mutant, consistent with microarray data. N. gonorrhoeae ntrX mutants had reduced capacity to survive inside primary cervical cells compared to the wild type, and although they retained the ability to form a biofilm, they exhibited reduced survival within the biofilm compared to wild-type cells, as indicated by LIVE/DEAD staining. Analyses of an ntrX mutant in a representative alphaproteobacterium, Rhodobacter capsulatus, showed that cytochrome oxidase activity was also reduced compared to that in the wild-type strain SB1003. Taken together, these data provide evidence that the NtrYX two-component system may be a key regulator in the expression of respiratory enzymes and, in particular, cytochrome c oxidase, across a wide range of proteobacteria, including a variety of bacterial pathogens.

SUBMITTER: Atack JM 

PROVIDER: S-EPMC3676050 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

Characterization of an ntrX mutant of Neisseria gonorrhoeae reveals a response regulator that controls expression of respiratory enzymes in oxidase-positive proteobacteria.

Atack John M JM   Srikhanta Yogitha N YN   Djoko Karrera Y KY   Welch Jessica P JP   Hasri Norain H M NH   Steichen Christopher T CT   Vanden Hoven Rachel N RN   Grimmond Sean M SM   Othman Dk Seti Maimonah Pg DS   Kappler Ulrike U   Apicella Michael A MA   Jennings Michael P MP   Edwards Jennifer L JL   McEwan Alastair G AG  

Journal of bacteriology 20130405 11


NtrYX is a sensor-histidine kinase/response regulator two-component system that has had limited characterization in a small number of Alphaproteobacteria. Phylogenetic analysis of the response regulator NtrX showed that this two-component system is extensively distributed across the bacterial domain, and it is present in a variety of Betaproteobacteria, including the human pathogen Neisseria gonorrhoeae. Microarray analysis revealed that the expression of several components of the respiratory ch  ...[more]

Similar Datasets

| S-EPMC4428587 | biostudies-literature
| S-EPMC178181 | biostudies-other
2016-05-24 | GSE73032 | GEO
2019-11-22 | GSE134959 | GEO
2017-02-09 | GSE60347 | GEO
| S-EPMC3942440 | biostudies-literature
| S-EPMC7499807 | biostudies-literature
2008-12-26 | GSE12686 | GEO
2009-02-27 | GSE14815 | GEO
| S-EPMC2848598 | biostudies-literature