Ontology highlight
ABSTRACT:
SUBMITTER: Nguyen TH
PROVIDER: S-EPMC3677097 | biostudies-literature | 2013 Jun
REPOSITORIES: biostudies-literature
Nguyen Thi Hoang Duong TH Li Jade J Galej Wojciech P WP Oshikane Hiroyuki H Newman Andrew J AJ Nagai Kiyoshi K
Structure (London, England : 1993) 20130601 6
The U5 small nuclear ribonucleoprotein particle (snRNP) helicase Brr2 disrupts the U4/U6 small nuclear RNA (snRNA) duplex and allows U6 snRNA to engage in an intricate RNA network at the active center of the spliceosome. Here, we present the structure of yeast Brr2 in complex with the Jab1/MPN domain of Prp8, which stimulates Brr2 activity. Contrary to previous reports, our crystal structure and mutagenesis data show that the Jab1/MPN domain binds exclusively to the N-terminal helicase cassette. ...[more]