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Effect of ex vivo culture conditions on immunosuppression by human mesenchymal stem cells.


ABSTRACT: A microarray analysis was performed to investigate whether ex vivo culture conditions affect the characteristics of MSCs. Gene expression profiles were mainly influenced by the level of cell confluence rather than initial seeding density. The analysis showed that 276 genes were upregulated and 230 genes downregulated in MSCs harvested at ~90% versus ~50% confluence (P < 0.05, FC > 2). The genes that were highly expressed in MSCs largely corresponded to chemotaxis, inflammation, and immune responses, indicating direct or indirect involvement in immunomodulatory functions. Specifically, PTGES and ULBP1 were up-regulated in MSCs harvested at high density. Treatment of MSCs with PTGES or ULBP1 siRNA reversed their inhibition of T-cell proliferation in vitro. The culture conditions such as cell confluence at harvest seem to be important for gene expression profile of MSCs; therefore, the results of this study may provide useful guidelines for the harvest of MSCs that can appropriately suppress the immune response.

SUBMITTER: Lee MW 

PROVIDER: S-EPMC3687591 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Effect of ex vivo culture conditions on immunosuppression by human mesenchymal stem cells.

Lee Myoung Woo MW   Kim Dae Seong DS   Ryu Somi S   Jang In Keun IK   Kim Hye Jin HJ   Yang Jin Mo JM   Lee Doo-Hoon DH   Lee Soo Hyun SH   Son Meong Hi MH   Cheuh Hee Won HW   Jung Hye Lim HL   Yoo Keon Hee KH   Sung Ki Woong KW   Koo Hong Hoe HH  

BioMed research international 20130604


A microarray analysis was performed to investigate whether ex vivo culture conditions affect the characteristics of MSCs. Gene expression profiles were mainly influenced by the level of cell confluence rather than initial seeding density. The analysis showed that 276 genes were upregulated and 230 genes downregulated in MSCs harvested at ~90% versus ~50% confluence (P < 0.05, FC > 2). The genes that were highly expressed in MSCs largely corresponded to chemotaxis, inflammation, and immune respon  ...[more]

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