Ontology highlight
ABSTRACT:
SUBMITTER: McClelland R
PROVIDER: S-EPMC2913780 | biostudies-literature | 2008 Dec
REPOSITORIES: biostudies-literature
McClelland Randall R Wauthier Eliane E Zhang Lili L Melhem Alaa A Schmelzer Eva E Barbier Claire C Reid Lola M LM
Tissue engineering. Part C, Methods 20081201 4
Human hepatic stem cells (hHpSCs), identifiable by a unique antigenic profile, have been isolated from human livers and established ex vivo under expansion conditions permissive for self-replication. The conditions consist of a substratum of type III collagen, ideally on Transwell inserts, and Kubota's medium, a serum-free medium developed for hepatic progenitors. Under these conditions the cells demonstrated a doubling time of approximately 24 h, generating at least a 16-fold increase in cell n ...[more]