Unknown

Dataset Information

0

Evidence for two different regulatory mechanisms linking replication and segregation of vibrio cholerae chromosome II.


ABSTRACT: Understanding the mechanisms that coordinate replication initiation with subsequent segregation of chromosomes is an important biological problem. Here we report two replication-control mechanisms mediated by a chromosome segregation protein, ParB2, encoded by chromosome II of the model multichromosome bacterium, Vibrio cholerae. We find by the ChIP-chip assay that ParB2, a centromere binding protein, spreads beyond the centromere and covers a replication inhibitory site (a 39-mer). Unexpectedly, without nucleation at the centromere, ParB2 could also bind directly to a related 39-mer. The 39-mers are the strongest inhibitors of chromosome II replication and they mediate inhibition by binding the replication initiator protein. ParB2 thus appears to promote replication by out-competing initiator binding to the 39-mers using two mechanisms: spreading into one and direct binding to the other. We suggest that both these are novel mechanisms to coordinate replication initiation with segregation of chromosomes.

SUBMITTER: Venkova-Canova T 

PROVIDER: S-EPMC3688505 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

Evidence for two different regulatory mechanisms linking replication and segregation of vibrio cholerae chromosome II.

Venkova-Canova Tatiana T   Baek Jong Hwan JH   Fitzgerald Peter C PC   Blokesch Melanie M   Chattoraj Dhruba K DK  

PLoS genetics 20130620 6


Understanding the mechanisms that coordinate replication initiation with subsequent segregation of chromosomes is an important biological problem. Here we report two replication-control mechanisms mediated by a chromosome segregation protein, ParB2, encoded by chromosome II of the model multichromosome bacterium, Vibrio cholerae. We find by the ChIP-chip assay that ParB2, a centromere binding protein, spreads beyond the centromere and covers a replication inhibitory site (a 39-mer). Unexpectedly  ...[more]

Similar Datasets

| S-EPMC3141006 | biostudies-literature
| S-EPMC3067663 | biostudies-literature
| S-EPMC3937223 | biostudies-literature
| S-EPMC1567695 | biostudies-literature
| S-EPMC3350373 | biostudies-literature
| S-EPMC5395669 | biostudies-literature
| S-EPMC1347293 | biostudies-literature
| S-EPMC2492486 | biostudies-literature
| S-EPMC4010829 | biostudies-other
| S-EPMC3021215 | biostudies-literature