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A quantitative ratiometric sensor for time-resolved analysis of auxin dynamics.

ABSTRACT: Time-resolved quantitative analysis of auxin-mediated processes in plant cells is as of yet limited. By applying a synergistic mammalian and plant synthetic biology approach, we have developed a novel ratiometric luminescent biosensor with wide applicability in the study of auxin metabolism, transport, and signalling. The sensitivity and kinetic properties of our genetically encoded biosensor open new perspectives for the analysis of highly complex auxin dynamics in plant growth and development.

SUBMITTER: Wend S

PROVIDER: S-EPMC3689175 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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A quantitative ratiometric sensor for time-resolved analysis of auxin dynamics.

Wend Sabrina S Dal Bosco Cristina C Kämpf Michael M MM Ren Fugang F Palme Klaus K Weber Wilfried W Dovzhenko Alexander A Zurbriggen Matias D MD

Scientific reports 20130101

Time-resolved quantitative analysis of auxin-mediated processes in plant cells is as of yet limited. By applying a synergistic mammalian and plant synthetic biology approach, we have developed a novel ratiometric luminescent biosensor with wide applicability in the study of auxin metabolism, transport, and signalling. The sensitivity and kinetic properties of our genetically encoded biosensor open new perspectives for the analysis of highly complex auxin dynamics in plant growth and development. ...[more]

PMID: 23787479

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