Browse
Submit Data
Databases
API
Help

Dataset Information

28 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Time-resolved analysis of photoluminescence at a single wavelength for ratiometric and multiplex biosensing and bioimaging.

ABSTRACT: Simultaneous analysis of luminescence signals of multiple probes can improve the accuracy and efficiency of biosensing and bioimaging. Analysis of multiple signals at different wavelengths usually suffers from spectral overlap, possible energy transfer, and difference in detection efficiency. Herein, we reported a polymeric luminescent probe, which was composed of a phenothiazine-based fluorescent compound and a phosphorescent iridium(iii) complex. Both luminophores emitted at around 600 nm but their luminescence lifetimes are 160 times different, allowing time-resolved independent analysis. As the fluorescence was enhanced in response to oxidation by hypochlorite and the phosphorescence was sensitive toward oxygen quenching, a four-dimensional relationship between luminescence intensity, fluorescence/phosphorescence ratio, hypochlorite concentration, and oxygen content was established. In cellular imaging, time-resolved photoluminescence imaging microscopy clearly showed the independent fluorescence response toward hypochlorite and phosphorescence response toward oxygen in separated time intervals. This work opens up a new idea for the development of multiplex biosensing and bioimaging.

SUBMITTER: Wu Q

PROVIDER: S-EPMC8386655 | biostudies-literature |

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Similar Datasets

Ratiometric upconversion nanothermometry with dual emission at the same wavelength decoded via a time-resolved technique.

Project description:The in vivo temperature monitoring of a microenvironment is significant in biology and nanomedicine research. Luminescent nanothermometry provides a noninvasive method of detecting the temperature in vivo with high sensitivity and high response speed. However, absorption and scattering in complex tissues limit the signal penetration depth and cause errors due to variation at different locations in vivo. In order to minimize these errors and monitor temperature in vivo, in the present work, we provided a strategy to fabricate a same-wavelength dual emission ratiometric upconversion luminescence nanothermometer based on a hybrid structure composed of upconversion emissive PbS quantum dots and Tm-doped upconversion nanoparticles. The ratiometric signal composed of two upconversion emissions working at the same wavelength, but different luminescent lifetimes, were decoded via a time-resolved technique. This nanothermometer improved the temperature monitoring ability and a thermal resolution and sensitivity of ~0.5 K and ~5.6% K-1 were obtained in vivo, respectively.

| S-EPMC6946702 | biostudies-literature

A wavelength-resolved ratiometric photoelectrochemical technique: design and sensing applications.

Project description:In this work, a wavelength-resolved ratiometric photoelectrochemical (WR-PEC) technique was investigated and employed to construct a new type of PEC sensor with good sensitivity and anti-interference ability. The WR-PEC hybrid photoelectrodes were stepwise assembled using semiconductor quantum dots (QDs) and photoactive dyes. Under continuous irradiation, the photocurrent-wavelength (I-λ) curves reveal the dependence of the photocurrent on the wavelength. By monitoring the ratios of the two different PEC peak values, a wavelength-resolved ratiometric strategy was realized. Using CdS QDs and methylene blue (MB) as photoactive models, a dual-anodic WR-PEC sensor was established for sensitive detection of Cu2+. This ratiometric strategy was identified to be based on the quenching effect of Cu2+ towards CdS QDs and enhancement of the MB photocurrent through catalytic oxidation of leuco-MB. Under continuous illumination from 400 nm to 800 nm at a 0.1 V bias potential, a WR-PEC sensor for Cu2+ was developed with a wide linear range and a detection limit of 0.37 nM. This WR-PEC had a greatly improved anti-interference ability in a complex environment, and showed acceptable stability. Moreover, using the CdS/magnesium phthalocyanine (MgPc) and CdTe/MgPc as photoelectrodes, anodic-cathodic and dual-cathodic WR-PEC sensors were established, respectively. The WR-PEC technique could serve as a novel concept for designing ratiometric or multi-channel PEC sensors.

| S-EPMC5580031 | biostudies-literature

A quantitative ratiometric sensor for time-resolved analysis of auxin dynamics.

Project description:Time-resolved quantitative analysis of auxin-mediated processes in plant cells is as of yet limited. By applying a synergistic mammalian and plant synthetic biology approach, we have developed a novel ratiometric luminescent biosensor with wide applicability in the study of auxin metabolism, transport, and signalling. The sensitivity and kinetic properties of our genetically encoded biosensor open new perspectives for the analysis of highly complex auxin dynamics in plant growth and development.

| S-EPMC3689175 | biostudies-literature

Time-Resolved Photoluminescence Microscopy for the Analysis of Semiconductor-Based Paint Layers.

Project description:In conservation, science semiconductors occur as the constituent matter of the so-called semiconductor pigments, produced following the Industrial Revolution and extensively used by modern painters. With recent research highlighting the occurrence of various degradation phenomena in semiconductor paints, it is clear that their detection by conventional optical fluorescence imaging and microscopy is limited by the complexity of historical painting materials. Here, we illustrate and prove the capabilities of time-resolved photoluminescence (TRPL) microscopy, equipped with both spectral and lifetime sensitivity at timescales ranging from nanoseconds to hundreds of microseconds, for the analysis of cross-sections of paint layers made of luminescent semiconductor pigments. The method is sensitive to heterogeneities within micro-samples and provides valuable information for the interpretation of the nature of the emissions in samples. A case study is presented on micro samples from a painting by Henri Matisse and serves to demonstrate how TRPL can be used to identify the semiconductor pigments zinc white and cadmium yellow, and to inform future investigations of the degradation of a cadmium yellow paint.

| S-EPMC5706282 | biostudies-literature

Effective Electron Temperature Measurement Using Time-Resolved Anti-Stokes Photoluminescence.

Project description:Anti-Stokes photoluminescence of metal nanoparticles, in which emitted photons have a higher energy than the incident photons, is an indicator of the temperature prevalent within a nanoparticle. Previous work has shown how to extract the temperature from a gold nanoparticle under continuous-wave monochromatic illumination. We extend the technique to pulsed illumination and introduce pump-probe anti-Stokes spectroscopy. This new technique enables us not only to measure an effective electron temperature in a gold nanoparticle (∼103 K under our conditions), but also to measure ultrafast dynamics of a pulse-excited electron population, through its effect on the photoluminescence, with subpicosecond time resolution. We measure the heating and cooling, all within picoseconds, of the electrons and find that, with our subpicosecond pulses, the highest apparent temperature is reached 0.6 ps before the maximum change in magnitude of the extinction signal.

| S-EPMC7457233 | biostudies-literature

Communicating Two States in Perovskite Revealed by Time-Resolved Photoluminescence Spectroscopy.

Project description:Organic-inorganic perovskite as a promising candidate for solar energy harvesting has attracted immense interest for its low-cost preparation and extremely high quantum efficiency. However, the fundamental understanding of the photophysics in perovskite remains elusive. In this work, we have revealed two distinct states in MAPbI3 thin films at low temperature through time-resolved photoluminescence spectroscopy (TRPL). In particular, we observed a photo-induced carrier injection from the high energy (HE) state to the low energy (LE) state which has a longer lifetime. The strong interaction between the two states, evidenced by the injection kinetics, can be sensitively controlled through the excitation power. Understanding of the interacting two-states not only sheds light on the long PL lifetime in perovskite but also helps to understand the different behavior of perovskite in response to different excitation power. Further efforts in modifying the low energy state could significantly improve the quantum efficiency and lead to novel application in optoelectronics based on perovskite.

| S-EPMC6220243 | biostudies-literature

Geometrical Patterning of Super-Hydrophobic Biosensing Transistors Enables Space and Time Resolved Analysis of Biological Mixtures.

Project description:PedotPSS is a conductive polymer that can be integrated into last generation Organic Electrochemical Transistor (OECT) devices for biological inspection, identification and analysis. While a variety of reports in literature demonstrated the chemical and biological sensitivity of these devices, still their ability in resolving complex mixtures remains controversial. Similar OECT devices display good time dynamics behavior but lack spatial resolution. In this work, we integratedPedotPSS with patterns of super-hydrophobic pillars in which a finite number of those pillars is independently controlled for site-selective measurement of a solution. We obtained a multifunctional, hierarchical OECT device that bridges the micro- to the nano-scales for specific, combined time and space resolved analysis of the sample. Due to super-hydrophobic surface properties, the biological species in the drop are driven by convection, diffusion, and the externally applied electric field: the balance/unbalance between these forces will cause the molecules to be transported differently within its volume depending on particle size thus realizing a size-selective separation. Within this framework, the separation and identification of two different molecules, namely Cetyl Trimethyl Ammonium Bromid (CTAB) and adrenaline, in a biological mixture have been demonstrated, showing that geometrical control at the micro-nano scale impart unprecedented selectivity to the devices.

| S-EPMC4709515 | biostudies-literature

Spectral and time-resolved photoluminescence of human platelets doped with platinum nanoparticles.

Project description:This paper describes a detailed study of spectral and time-resolved photoprocesses in human platelets and their complexes with platinum (Pt) nanoparticles (NPs). Fluorescence, quantum yield, and platelet amino acid lifetime changes in the presence and without femtosecond ablated platinum NPs have been studied. Fluorescence spectroscopy analysis of main fluorescent amino acids and their residues (tyrosine (Tyr), tryptophan (Trp), and phenylalanine (Phe)) belonging to the platelet membrane have been performed. The possibility of energy transfer between Pt NPs and the platelet membrane has been revealed. Förster Resonance Energy Transfer (FRET) model was used to perform the quantitative evaluation of energy transfer parameters. The prospects of Pt NPs usage deals with quenching-based sensing for pathology's based on platelet conformations as cardiovascular diseases have been demonstrated.

| S-EPMC8409683 | biostudies-literature

Multiplex Photoluminescent Silicon Nanoprobe for Diagnostic Bioimaging and Intracellular Analysis.

Project description:Herein, a label-free multiplex photoluminescent silicon nanoprobe (PLSN-probe) is introduced as a potential substitute for quantum dots (QDs) in bioimaging. An inherently non-photoluminescent silicon substrate is altered to create the PLSN-probe, to overcome the major drawbacks of presently available QDs. Additionally, crystallinity alterations of the multiplane crystalline PLSN-probes lead to broad absorption and multiplex fluorescence emissions, which are attributed to the simultaneous existence of multiple crystal planes. The PLSN-probe not only demonstrates unique optical properties that can be exploited for bioimaging but also exhibits cell-selective uptake that allows the differentiation and diagnosis of HeLa and fibroblast cells. Moreover, multiplex emissions of the PLSN-probe illuminate different organelles such as the nucleus, nucleolemma, and cytoskeleton, depending on size-based preferential uptake by the cell organs. This in vitro study reveals that cancerous HeLa cells have a higher propensity for taking up the PLSN-probe compared to fibroblast cells, allowing the diagnosis of cancerous HeLa cells. Additionally, the fluorescence intensity per unit area of the cell is found to be a reliable means for distinguishing between dead and healthy cells. It is anticipated that the multifunctionality of the PLSN-probes will lead to better insight into the use of such probes for bioimaging and diagnosis applications.

| S-EPMC5867044 | biostudies-literature

Multiplex bioimaging of single-cell spatial profiles for precision cancer diagnostics and therapeutics.

Project description:Cancers exhibit functional and structural diversity in distinct patients. In this mass, normal and malignant cells create tumor microenvironment that is heterogeneous among patients. A residue from primary tumors leaks into the bloodstream as cell clusters and single cells, providing clues about disease progression and therapeutic response. The complexity of these hierarchical microenvironments needs to be elucidated. Although tumors comprise ample cell types, the standard clinical technique is still the histology that is limited to a single marker. Multiplexed imaging technologies open new directions in pathology. Spatially resolved proteomic, genomic, and metabolic profiles of human cancers are now possible at the single-cell level. This perspective discusses spatial bioimaging methods to decipher the cascade of microenvironments in solid and liquid biopsies. A unique synthesis of top-down and bottom-up analysis methods is presented. Spatial multi-omics profiles can be tailored to precision oncology through artificial intelligence. Data-driven patient profiling enables personalized medicine and beyond.

| S-EPMC7195402 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data