Ontology highlight
ABSTRACT:
SUBMITTER: Clijsters L
PROVIDER: S-EPMC3691463 | biostudies-literature | 2013 Jun
REPOSITORIES: biostudies-literature
The Journal of cell biology 20130617 7
DNA replication depends on a preceding licensing event by Cdt1 and Cdc6. In animal cells, relicensing after S phase but before mitosis is prevented by the Cdt1 inhibitor geminin and mitotic cyclin activity. Here, we show that geminin, like cyclin B1 and securin, is a bona fide target of the spindle checkpoint and APC/C(Cdc20). Cyclin B1 and geminin are degraded simultaneously during metaphase, which directs Cdt1 accumulation on segregating sister chromatids. Subsequent activation of APC/C(Cdh1) ...[more]