Dataset Information

Proteomic analysis of energy metabolism and signal transduction in irradiated melanoma cells.

ABSTRACT: AIM:To analyze proteomic and signal transduction alterations in irradiated melanoma cells. METHODS:We combined stable isotope labeling with amino acids in cell culture (SILAC) with highly sensitive shotgun tandem mass spectrometry (MS) to create an efficient approach for protein quantification. Protein-protein interaction was used to analyze relationships among proteins. RESULTS:Energy metabolism protein levels were significantly different in glycolysis and not significantly different in oxidative phosphorylation after irradiation. Conversely, tumor suppressor proteins related to cell growth and development were downregulated, and those related to cell death and cell cycle were upregulated in irradiated cells. CONCLUSION:Our results indicate that irradiation induces differential expression of the 29 identified proteins closely related to cell survival, cell cycle arrest, and growth inhibition. The data may provide new insights into the pathogenesis of uveal melanoma and guide appropriate radiotherapy.

SUBMITTER: Yan LB

PROVIDER: S-EPMC3693007 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Proteomic analysis of energy metabolism and signal transduction in irradiated melanoma cells.

Yan Lu-Bin LB Shi Kai K Bing Zhi-Tong ZT Sun Yi-Lan YL Shen Yang Y

International journal of ophthalmology 20130618 3

<h4>Aim</h4>To analyze proteomic and signal transduction alterations in irradiated melanoma cells.<h4>Methods</h4>We combined stable isotope labeling with amino acids in cell culture (SILAC) with highly sensitive shotgun tandem mass spectrometry (MS) to create an efficient approach for protein quantification. Protein-protein interaction was used to analyze relationships among proteins.<h4>Results</h4>Energy metabolism protein levels were significantly different in glycolysis and not significantl ...[more]

PMID: 23826520

Similar Datasets

Project description:Parkinson disease is mainly characterized by the degeneration of dopaminergic neurons in the central nervous system, including the retina. Different interrelated molecular mechanisms underlying Parkinson disease-associated neuronal death have been put forward in the brain, including oxidative stress and mitochondrial dysfunction. Systemic injection of the proneurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) to monkeys elicits the appearance of a parkinsonian syndrome, including morphological and functional impairments in the retina. However, the intracellular events leading to derangement of dopaminergic and other retinal neurons in MPTP-treated animal models have not been so far investigated. Here we have used a comparative proteomics approach to identify proteins differentially expressed in the retina of MPTP-treated monkeys. Proteins were solubilized from the neural retinas of control and MPTP-treated animals, labelled separately with two different cyanine fluorophores and run pairwise on 2D DIGE gels. Out of >700 protein spots resolved and quantified, 36 were found to exhibit statistically significant differences in their expression levels, of at least ± 1.4-fold, in the parkinsonian monkey retina compared with controls. Most of these spots were excised from preparative 2D gels, trypsinized and subjected to MALDI-TOF MS and LC-MS/MS analyses. Data obtained were used for protein sequence database interrogation, and 15 different proteins were successfully identified, of which 13 were underexpressed and 2 overexpressed. These proteins were involved in key cellular functional pathways such as glycolysis and mitochondrial electron transport, neuronal protection against stress and survival, and phototransduction processes. These functional categories underscore that alterations in energy metabolism, neuroprotective mechanisms and signal transduction are involved in MPTP-induced neuronal degeneration in the retina, in similarity to mechanisms thought to underlie neuronal death in the Parkinson's diseased brain and neurodegenerative diseases of the retina proper.

Dataset Information

Proteomic analysis of energy metabolism and signal transduction in irradiated melanoma cells.

Publications

Proteomic analysis of energy metabolism and signal transduction in irradiated melanoma cells.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets