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A simple tool to improve pluripotent stem cell differentiation.

ABSTRACT: We describe a method to help overcome restrictions on the differentiation propensities of human pluripotent stem cells. Culturing pluripotent stem cells in dimethylsulfoxide (DMSO) activates the retinoblastoma protein, increases the proportion of cells in the early G1 phase of the cell cycle and, in more than 25 embryonic and induced pluripotent stem cell lines, improves directed differentiation into multiple lineages. DMSO treatment also improves differentiation into terminal cell types in several cell lines.

SUBMITTER: Chetty S

PROVIDER: S-EPMC3694177 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature

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A simple tool to improve pluripotent stem cell differentiation.

Chetty Sundari S Pagliuca Felicia Walton FW Honore Christian C Kweudjeu Anastasie A Rezania Alireza A Melton Douglas A DA

Nature methods 20130414 6

We describe a method to help overcome restrictions on the differentiation propensities of human pluripotent stem cells. Culturing pluripotent stem cells in dimethylsulfoxide (DMSO) activates the retinoblastoma protein, increases the proportion of cells in the early G1 phase of the cell cycle and, in more than 25 embryonic and induced pluripotent stem cell lines, improves directed differentiation into multiple lineages. DMSO treatment also improves differentiation into terminal cell types in seve ...[more]

PMID: 23584186

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