Ontology highlight
ABSTRACT:
SUBMITTER: Guadix JA
PROVIDER: S-EPMC5785703 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
Guadix Juan Antonio JA Orlova Valeria V VV Giacomelli Elisa E Bellin Milena M Ribeiro Marcelo C MC Mummery Christine L CL Pérez-Pomares José M JM Passier Robert R
Stem cell reports 20171122 6
Human pluripotent stem cells (hPSCs) are widely used to study cardiovascular cell differentiation and function. Here, we induced differentiation of hPSCs (both embryonic and induced) to proepicardial/epicardial progenitor cells that cover the heart during development. Addition of retinoic acid (RA) and bone morphogenetic protein 4 (BMP4) promoted expression of the mesodermal marker PDGFRα, upregulated characteristic (pro)epicardial progenitor cell genes, and downregulated transcription of myocar ...[more]