Ontology highlight
ABSTRACT:
SUBMITTER: Chen DH
PROVIDER: S-EPMC3695626 | biostudies-literature | 2013 Jun
REPOSITORIES: biostudies-literature
Chen Dong-Hua DH Madan Damian D Weaver Jeremy J Lin Zong Z Schröder Gunnar F GF Chiu Wah W Rye Hays S HS
Cell 20130601 6
The GroEL/ES chaperonin system is required for the assisted folding of many proteins. How these substrate proteins are encapsulated within the GroEL-GroES cavity is poorly understood. Using symmetry-free, single-particle cryo-electron microscopy, we have characterized a chemically modified mutant of GroEL (EL43Py) that is trapped at a normally transient stage of substrate protein encapsulation. We show that the symmetric pattern of the GroEL subunits is broken as the GroEL cis-ring apical domain ...[more]