Ontology highlight
ABSTRACT:
SUBMITTER: Lin Z
PROVIDER: S-EPMC3759401 | biostudies-literature | 2004 Oct
REPOSITORIES: biostudies-literature
Molecular cell 20041001 1
The GroEL-GroES chaperonin system is required for the assisted folding of many essential proteins. The precise nature of this assistance remains unclear, however. Here we show that denatured RuBisCO from Rhodospirillum rubrum populates a stable, nonaggregating, and kinetically trapped monomeric state at low temperature. Productive folding of this nonnative intermediate is fully dependent on GroEL, GroES, and ATP. Reactivation of the trapped RuBisCO monomer proceeds through a series of GroEL-indu ...[more]