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Rotational mobility of single molecules affects localization accuracy in super-resolution fluorescence microscopy.

ABSTRACT: The asymmetric nature of single-molecule (SM) dipole emission patterns limits the accuracy of position determination in localization-based super-resolution fluorescence microscopy. The degree of mislocalization depends highly on the rotational mobility of SMs; only for SMs rotating within a cone half angle ? > 60° can mislocalization errors be bounded to ?10 nm. Simulations demonstrate how low or high rotational mobility can cause resolution degradation or distortion in super-resolution reconstructions.

SUBMITTER: Lew MD 

PROVIDER: S-EPMC3696529 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature

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Rotational mobility of single molecules affects localization accuracy in super-resolution fluorescence microscopy.

Lew Matthew D MD   Backlund Mikael P MP   Moerner W E WE  

Nano letters 20130206 9

The asymmetric nature of single-molecule (SM) dipole emission patterns limits the accuracy of position determination in localization-based super-resolution fluorescence microscopy. The degree of mislocalization depends highly on the rotational mobility of SMs; only for SMs rotating within a cone half angle α > 60° can mislocalization errors be bounded to ≤10 nm. Simulations demonstrate how low or high rotational mobility can cause resolution degradation or distortion in super-resolution reconstr  ...[more]

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