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Ultrahigh accuracy imaging modality for super-localization microscopy.

ABSTRACT: Super-localization microscopy encompasses techniques that depend on the accurate localization of individual molecules from generally low-light images. The obtainable localization accuracies, however, are ultimately limited by the image detector's pixelation and noise. We present the ultrahigh accuracy imaging modality (UAIM), which allows users to obtain accuracies approaching the accuracy that is achievable only in the absence of detector pixelation and noise, and which we found can experimentally provide a >200% accuracy improvement over conventional low-light imaging.

SUBMITTER: Chao J

PROVIDER: S-EPMC3833351 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature

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Ultrahigh accuracy imaging modality for super-localization microscopy.

Chao Jerry J Ram Sripad S Ward E Sally ES Ober Raimund J RJ

Nature methods 20130303 4

Super-localization microscopy encompasses techniques that depend on the accurate localization of individual molecules from generally low-light images. The obtainable localization accuracies, however, are ultimately limited by the image detector's pixelation and noise. We present the ultrahigh accuracy imaging modality (UAIM), which allows users to obtain accuracies approaching the accuracy that is achievable only in the absence of detector pixelation and noise, and which we found can experimenta ...[more]

PMID: 23455923

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