Trehalose-enhanced isolation of neuronal sub-types from adult mouse brain.
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ABSTRACT:
SUBMITTER: Saxena A
PROVIDER: S-EPMC3696583 | biostudies-literature | 2012 Jun
REPOSITORIES: biostudies-literature
Publications
Trehalose-enhanced isolation of neuronal sub-types from adult mouse brain.
Saxena Alka A Wagatsuma Akiko A Noro Yukihiko Y Kuji Takenobu T Asaka-Oba Atsuko A Watahiki Akira A Gurnot Cecile C Fagiolini Michela M Hensch Takao K TK Carninci Piero P
BioTechniques 20120601 6
Efficient isolation of specific, intact, living neurons from the adult brain is problematic due to the complex nature of the extracellular matrix consolidating the neuronal network. Here, we present significant improvements to the protocol for isolation of pure populations of neurons from mature postnatal mouse brain using fluorescence activated cell sorting (FACS). The 10-fold increase in cell yield enables cell-specific transcriptome analysis by protocols such as nanoCAGE and RNA seq. ...[more]
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