Ontology highlight
ABSTRACT:
SUBMITTER: Straimer J
PROVIDER: S-EPMC3697006 | biostudies-literature | 2012 Oct
REPOSITORIES: biostudies-literature
Straimer Judith J Lee Marcus C S MC Lee Andrew H AH Zeitler Bryan B Williams April E AE Pearl Jocelynn R JR Zhang Lei L Rebar Edward J EJ Gregory Philip D PD Llinás Manuel M Urnov Fyodor D FD Fidock David A DA
Nature methods 20120826 10
Malaria afflicts over 200 million people worldwide, and its most lethal etiologic agent, Plasmodium falciparum, is evolving to resist even the latest-generation therapeutics. Efficient tools for genome-directed investigations of P. falciparum-induced pathogenesis, including drug-resistance mechanisms, are clearly required. Here we report rapid and targeted genetic engineering of this parasite using zinc-finger nucleases (ZFNs) that produce a double-strand break in a user-defined locus and trigge ...[more]