Dataset Information

Integration of lyoplate based flow cytometry and computational analysis for standardized immunological biomarker discovery.

ABSTRACT: Discovery of novel immune biomarkers for monitoring of disease prognosis and response to therapy in immune-mediated inflammatory diseases is an important unmet clinical need. Here, we establish a novel framework for immunological biomarker discovery, comparing a conventional (liquid) flow cytometry platform (CFP) and a unique lyoplate-based flow cytometry platform (LFP) in combination with advanced computational data analysis. We demonstrate that LFP had higher sensitivity compared to CFP, with increased detection of cytokines (IFN-? and IL-10) and activation markers (Foxp3 and CD25). Fluorescent intensity of cells stained with lyophilized antibodies was increased compared to cells stained with liquid antibodies. LFP, using a plate loader, allowed medium-throughput processing of samples with comparable intra- and inter-assay variability between platforms. Automated computational analysis identified novel immunophenotypes that were not detected with manual analysis. Our results establish a new flow cytometry platform for standardized and rapid immunological biomarker discovery with wide application to immune-mediated diseases.

SUBMITTER: Villanova F

PROVIDER: S-EPMC3701052 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Integration of lyoplate based flow cytometry and computational analysis for standardized immunological biomarker discovery.

Villanova Federica F Di Meglio Paola P Inokuma Margaret M Aghaeepour Nima N Perucha Esperanza E Mollon Jennifer J Nomura Laurel L Hernandez-Fuentes Maria M Cope Andrew A Prevost A Toby AT Heck Susanne S Maino Vernon V Lord Graham G Brinkman Ryan R RR Nestle Frank O FO

PloS one 20130703 7

Discovery of novel immune biomarkers for monitoring of disease prognosis and response to therapy in immune-mediated inflammatory diseases is an important unmet clinical need. Here, we establish a novel framework for immunological biomarker discovery, comparing a conventional (liquid) flow cytometry platform (CFP) and a unique lyoplate-based flow cytometry platform (LFP) in combination with advanced computational data analysis. We demonstrate that LFP had higher sensitivity compared to CFP, with ...[more]

PMID: 23843942

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Integration of lyoplate based flow cytometry and computational analysis for standardized immunological biomarker discovery.

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Integration of lyoplate based flow cytometry and computational analysis for standardized immunological biomarker discovery.

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