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RNA SHAPE analysis in living cells.

ABSTRACT: RNA structure has important roles in practically every facet of gene regulation, but the paucity of in vivo structural probes limits current understanding. Here we design, synthesize and demonstrate two new chemical probes that enable selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE) in living cells. RNA structures in human, mouse, fly, yeast and bacterial cells are read out at single-nucleotide resolution, revealing tertiary contacts and RNA-protein interactions.

SUBMITTER: Spitale RC

PROVIDER: S-EPMC3706714 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature

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RNA SHAPE analysis in living cells.

Spitale Robert C RC Crisalli Pete P Flynn Ryan A RA Torre Eduardo A EA Kool Eric T ET Chang Howard Y HY

Nature chemical biology 20121125 1

RNA structure has important roles in practically every facet of gene regulation, but the paucity of in vivo structural probes limits current understanding. Here we design, synthesize and demonstrate two new chemical probes that enable selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE) in living cells. RNA structures in human, mouse, fly, yeast and bacterial cells are read out at single-nucleotide resolution, revealing tertiary contacts and RNA-protein interactions. ...[more]

PMID: 23178934

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