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NKT cell-TCR expression activates conventional T cells in vivo, but is largely dispensable for mature NKT cell biology.

ABSTRACT: Natural killer T (NKT) cell development depends on recognition of self-glycolipids via their semi-invariant V?14i-TCR. However, to what extent TCR-mediated signals determine identity and function of mature NKT cells remains incompletely understood. To address this issue, we developed a mouse strain allowing conditional V?14i-TCR expression from within the endogenous Tcr? locus. We demonstrate that naïve T cells are activated upon replacement of their endogenous TCR repertoire with V?14i-restricted TCRs, but they do not differentiate into NKT cells. On the other hand, induced TCR ablation on mature NKT cells did not affect their lineage identity, homeostasis, or innate rapid cytokine secretion abilities. We therefore propose that peripheral NKT cells become unresponsive to and thus are independent of their autoreactive TCR.

SUBMITTER: Vahl JC

PROVIDER: S-EPMC3708704 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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NKT cell-TCR expression activates conventional T cells in vivo, but is largely dispensable for mature NKT cell biology.

Vahl J Christoph JC Heger Klaus K Knies Nathalie N Hein Marco Y MY Boon Louis L Yagita Hideo H Polic Bojan B Schmidt-Supprian Marc M

PLoS biology 20130618 6

Natural killer T (NKT) cell development depends on recognition of self-glycolipids via their semi-invariant Vα14i-TCR. However, to what extent TCR-mediated signals determine identity and function of mature NKT cells remains incompletely understood. To address this issue, we developed a mouse strain allowing conditional Vα14i-TCR expression from within the endogenous Tcrα locus. We demonstrate that naïve T cells are activated upon replacement of their endogenous TCR repertoire with Vα14i-restrict ...[more]

PMID: 23853545

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