Ontology highlight
ABSTRACT:
SUBMITTER: Harms MJ
PROVIDER: S-EPMC3710831 | biostudies-literature | 2013 Jul
REPOSITORIES: biostudies-literature
Harms Michael J MJ Eick Geeta N GN Goswami Devrishi D Colucci Jennifer K JK Griffin Patrick R PR Ortlund Eric A EA Thornton Joseph W JW
Proceedings of the National Academy of Sciences of the United States of America 20130624 28
The genetic and biophysical mechanisms by which new protein functions evolve is a central question in evolutionary biology, biochemistry, and biophysics. Of particular interest is whether major shifts in protein function are caused by a few mutations of large effect and, if they are, the mechanisms that mediate these changes. Here we combine ancestral protein reconstruction with genetic manipulation and explicit studies of protein structure and dynamics to dissect an ancient and discrete shift i ...[more]