Ontology highlight
ABSTRACT:
SUBMITTER: Gokcen A
PROVIDER: S-EPMC3711985 | biostudies-literature | 2013 Apr
REPOSITORIES: biostudies-literature
Virulence 20130128 3
The production of extracellular poly-β-1,6-N-acetyl-D-glucosamine (PNAG) by Staphylococcus epidermidis is the principal determinant of biofilm formation on indwelling medical devices. Enzymes that degrade PNAG therefore provide an attractive strategy for biofilm removal and for the manufacture of biofilm-resistant coatings. Here we present methods that allow the identification of PNAG-degrading enzymes with the ability to detach biofilms. Our protocol includes the preparation of soluble PNAG fro ...[more]