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Cryptosporidium hominis infection diagnosed by real-time PCR-RFLP.

ABSTRACT: There are approximately 20 known species of the genus Cryptosporidium, and among these, 8 infect immunocompetent or immunocompromised humans. C. hominis and C. parvum most commonly infect humans. Differentiating between them is important for evaluating potential sources of infection. We report here the development of a simple and accurate real-time PCR-based restriction fragment length polymorphism (RFLP) method to distinguish between C. parvum and C. hominis. Using the CP2 gene as the target, we found that both Cryptosporidium species yielded 224 bp products. In the subsequent RFLP method using TaqI, 2 bands (99 and 125 bp) specific to C. hominis were detected. Using this method, we detected C. hominis infection in 1 of 21 patients with diarrhea, suggesting that this method could facilitate the detection of C. hominis infections.

SUBMITTER: Cheun HI

PROVIDER: S-EPMC3712111 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature

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Cryptosporidium hominis infection diagnosed by real-time PCR-RFLP.

Cheun Hyeng-Il HI Kim Kyungjin K Yoon Sejoung S Lee Won-Ja WJ Park Woo-Yoon WY Sim Seobo S Yu Jae-Ran JR

The Korean journal of parasitology 20130630 3

There are approximately 20 known species of the genus Cryptosporidium, and among these, 8 infect immunocompetent or immunocompromised humans. C. hominis and C. parvum most commonly infect humans. Differentiating between them is important for evaluating potential sources of infection. We report here the development of a simple and accurate real-time PCR-based restriction fragment length polymorphism (RFLP) method to distinguish between C. parvum and C. hominis. Using the CP2 gene as the target, w ...[more]

PMID: 23864748

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