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Evaluation of a modular multiplex-PCR methicillin-resistant Staphylococcus aureus detection assay adapted for mecC detection.

ABSTRACT: A mecC (mecALGA251)-adapted multiplex PCR-based methicillin-resistant Staphylococcus aureus (MRSA) detection assay was evaluated using an international, spa-typed Staphylococcus aureus collection comprising 51 mecC-positive MRSA, 240 mecA-positive MRSA, and 50 mecA- and mecC-negative methicillin-susceptible S. aureus (MSSA) isolates. The assay showed 100% sensitivity and specificity for S. aureus species identification as well as for mecA and mecC detection.

SUBMITTER: Becker K

PROVIDER: S-EPMC3716076 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature

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Evaluation of a modular multiplex-PCR methicillin-resistant Staphylococcus aureus detection assay adapted for mecC detection.

Becker Karsten K Larsen Anders R AR Skov Robert L RL Paterson Gavin K GK Holmes Mark A MA Sabat Artur J AJ Friedrich Alexander W AW Köck Robin R Peters Georg G Kriegeskorte André A

Journal of clinical microbiology 20130320 6

A mecC (mecALGA251)-adapted multiplex PCR-based methicillin-resistant Staphylococcus aureus (MRSA) detection assay was evaluated using an international, spa-typed Staphylococcus aureus collection comprising 51 mecC-positive MRSA, 240 mecA-positive MRSA, and 50 mecA- and mecC-negative methicillin-susceptible S. aureus (MSSA) isolates. The assay showed 100% sensitivity and specificity for S. aureus species identification as well as for mecA and mecC detection. ...[more]

PMID: 23515551

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