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Development of a single-reaction multiplex PCR toxin typing assay for Staphylococcus aureus strains.

ABSTRACT: We describe here the development of a single-reaction multiplex PCR assay for the enterotoxin genes from Staphylococcus aureus that utilizes a universal toxin gene primer in combination with toxin-specific primers to amplify characteristic toxin gene products. In combination with a new DNA purification method, the assay can detect enterotoxin genes A to E from a pure culture within 3 to 4 h. The test was used to characterize a diverse set of environmental S. aureus isolates, and a 99% correlation with toxin typing using standard immunological tests was found. The design of the assay allows it to be extended to include both newly characterized and as-yet-unknown toxin genes.

SUBMITTER: Sharma NK

PROVIDER: S-EPMC91991 | biostudies-literature | 2000 Apr

REPOSITORIES: biostudies-literature

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Development of a single-reaction multiplex PCR toxin typing assay for Staphylococcus aureus strains.

Sharma N K NK Rees C E CE Dodd C E CE

Applied and environmental microbiology 20000401 4

We describe here the development of a single-reaction multiplex PCR assay for the enterotoxin genes from Staphylococcus aureus that utilizes a universal toxin gene primer in combination with toxin-specific primers to amplify characteristic toxin gene products. In combination with a new DNA purification method, the assay can detect enterotoxin genes A to E from a pure culture within 3 to 4 h. The test was used to characterize a diverse set of environmental S. aureus isolates, and a 99% correlatio ...[more]

PMID: 10742210

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