Synergetic effect of yeast cell-surface expression of cellulase and expansin-like protein on direct ethanol production from cellulose.

ABSTRACT:

Background

Numerous studies have examined the direct fermentation of cellulosic materials by cellulase-expressing yeast; however, ethanol productivity in these systems has not yet reached an industrial level. Certain microorganisms, such as the cellulolytic fungus Trichoderma reesei, produce expansin-like proteins, which have a cellulose-loosening effect that may increase the breakdown of cellulose. Here, to improve the direct conversion of cellulose to ethanol, yeast Saccharomyces cerevisiae co-displaying cellulase and expansin-like protein on the cell surface were constructed and examined for direct ethanol fermentation performance.

Results

The cellulase and expansin-like protein co-expressing strain showed 246 mU/g-wet cell of phosphoric acid swollen cellulose (PASC) degr

SUBMITTER: Nakatani Y 

PROVIDER: S-EPMC3716962 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature