Ontology highlight
ABSTRACT:
SUBMITTER: Li H
PROVIDER: S-EPMC3718136 | biostudies-literature | 2013 Jul
REPOSITORIES: biostudies-literature
Li Huang H Wang Zhijia Z Zhou Xuexia X Cheng Yuanming Y Xie Zhiqin Z Manley James L JL Feng Ying Y
Proceedings of the National Academy of Sciences of the United States of America 20130701 29
Splicing of mRNA precursors consists of two steps that are almost invariably tightly coupled to facilitate efficient generation of spliced mRNA. However, we described previously a splicing substrate that is completely blocked after the first step. We have now investigated the basis for this unusual second-step inhibition and unexpectedly elucidated two independent mechanisms. One involves a stem-loop structure located downstream of the 3'splice site, and the other involves an exonic splicing sil ...[more]