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A serine-substituted P450 catalyzes highly efficient carbene transfer to olefins in vivo.

ABSTRACT: Whole-cell catalysts for non-natural chemical reactions will open new routes to sustainable production of chemicals. We designed a cytochrome 'P411' with unique serine-heme ligation that catalyzes efficient and selective olefin cyclopropanation in intact Escherichia coli cells. The mutation C400S in cytochrome P450(BM3) gives a signature ferrous CO Soret peak at 411 nm, abolishes monooxygenation activity, raises the resting-state Fe(III)-to-Fe(II) reduction potential and substantially improves NAD(P)H-driven activity.

SUBMITTER: Coelho PS

PROVIDER: S-EPMC3720782 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature

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A serine-substituted P450 catalyzes highly efficient carbene transfer to olefins in vivo.

Coelho Pedro S PS Wang Z Jane ZJ Ener Maraia E ME Baril Stefanie A SA Kannan Arvind A Arnold Frances H FH Brustad Eric M EM

Nature chemical biology 20130623 8

Whole-cell catalysts for non-natural chemical reactions will open new routes to sustainable production of chemicals. We designed a cytochrome 'P411' with unique serine-heme ligation that catalyzes efficient and selective olefin cyclopropanation in intact Escherichia coli cells. The mutation C400S in cytochrome P450(BM3) gives a signature ferrous CO Soret peak at 411 nm, abolishes monooxygenation activity, raises the resting-state Fe(III)-to-Fe(II) reduction potential and substantially improves N ...[more]

PMID: 23792734

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