Unknown

Dataset Information

0

Immunoglobulin-like domain containing receptor 1 mediates fat-stimulated cholecystokinin secretion.


ABSTRACT: Cholecystokinin (CCK) is a satiety hormone produced by discrete enteroendocrine cells scattered among absorptive cells of the small intestine. CCK is released into blood following a meal; however, the mechanisms inducing hormone secretion are largely unknown. Ingested fat is the major stimulant of CCK secretion. We recently identified a novel member of the lipoprotein remnant receptor family known as immunoglobulin-like domain containing receptor 1 (ILDR1) in intestinal CCK cells and postulated that this receptor conveyed the signal for fat-stimulated CCK secretion. In the intestine, ILDR1 is expressed exclusively in CCK cells. Orogastric administration of fatty acids elevated blood levels of CCK in wild-type mice but not Ildr1-deficient mice, although the CCK secretory response to trypsin inhibitor was retained. The uptake of fluorescently labeled lipoproteins in ILDR1-transfected CHO cells and release of CCK from isolated intestinal cells required a unique combination of fatty acid plus HDL. CCK secretion secondary to ILDR1 activation was associated with increased [Ca2+]i, consistent with regulated hormone release. These findings demonstrate that ILDR1 regulates CCK release through a mechanism dependent on fatty acids and lipoproteins and that absorbed fatty acids regulate gastrointestinal hormone secretion.

SUBMITTER: Chandra R 

PROVIDER: S-EPMC3726170 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature

altmetric image

Publications

Immunoglobulin-like domain containing receptor 1 mediates fat-stimulated cholecystokinin secretion.

Chandra Rashmi R   Wang Yu Y   Shahid Rafiq A RA   Vigna Steven R SR   Freedman Neil J NJ   Liddle Rodger A RA  

The Journal of clinical investigation 20130725 8


Cholecystokinin (CCK) is a satiety hormone produced by discrete enteroendocrine cells scattered among absorptive cells of the small intestine. CCK is released into blood following a meal; however, the mechanisms inducing hormone secretion are largely unknown. Ingested fat is the major stimulant of CCK secretion. We recently identified a novel member of the lipoprotein remnant receptor family known as immunoglobulin-like domain containing receptor 1 (ILDR1) in intestinal CCK cells and postulated  ...[more]

Similar Datasets

2013-05-23 | E-GEOD-47196 | biostudies-arrayexpress
2013-05-23 | GSE47196 | GEO
| S-EPMC4717904 | biostudies-literature
| S-EPMC1479700 | biostudies-literature
| S-EPMC3286985 | biostudies-literature
| S-EPMC3724619 | biostudies-literature
| S-EPMC2645526 | biostudies-literature
| S-EPMC3346071 | biostudies-literature
2022-10-13 | PXD035020 | Pride
2017-05-31 | PXD003336 | Pride