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CO?-based in-line phase contrast imaging of small intestine in mice.

ABSTRACT: The objective of this study was to explore the potential of CO? single contrast in-line phase contrast imaging (PCI) for pre-clinical small intestine investigation. The absorption and phase contrast images of CO? gas production were attained and compared. A further increase in image contrast was observed in PCI. Compared with CO?-based absorption contrast imaging (ACI), CO?-based PCI significantly enhanced the detection of mucosal microstructures, such as pits and folds. The CO?-based PCI could provide sufficient image contrast for clearly showing the intestinal mucosa in living mice without using barium. We concluded that CO?-based PCI might be a novel and promising imaging method for future studies of gastrointestinal disorders.

SUBMITTER: Tang R

PROVIDER: S-EPMC3727055 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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CO₂-based in-line phase contrast imaging of small intestine in mice.

Tang Rongbiao R Li Wei-Xia WX Huang Wei W Yan Fuhua F Chai Wei-Min WM Yang Guo-Yuan GY Chen Ke-Min KM

Scientific reports 20130101

The objective of this study was to explore the potential of CO₂ single contrast in-line phase contrast imaging (PCI) for pre-clinical small intestine investigation. The absorption and phase contrast images of CO₂ gas production were attained and compared. A further increase in image contrast was observed in PCI. Compared with CO₂-based absorption contrast imaging (ACI), CO₂-based PCI significantly enhanced the detection of mucosal microstructures, such as pits and folds. The CO₂-based PCI could ...[more]

PMID: 23896957

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Project description:This paper concerns the feasibility of x-ray differential phase contrast (DPC) tomosynthesis imaging using a grating-based DPC benchtop experimental system, which is equipped with a commercial digital flat-panel detector and a medical-grade rotating-anode x-ray tube. An extensive system characterization was performed to quantify its imaging performance.The major components of the benchtop system include a diagnostic x-ray tube with a 1.0 mm nominal focal spot size, a flat-panel detector with 96 μm pixel pitch, a sample stage that rotates within a limited angular span of ± 30°, and a Talbot-Lau interferometer with three x-ray gratings. A total of 21 projection views acquired with 3° increments were used to reconstruct three sets of tomosynthetic image volumes, including the conventional absorption contrast tomosynthesis image volume (AC-tomo) reconstructed using the filtered-backprojection (FBP) algorithm with the ramp kernel, the phase contrast tomosynthesis image volume (PC-tomo) reconstructed using FBP with a Hilbert kernel, and the differential phase contrast tomosynthesis image volume (DPC-tomo) reconstructed using the shift-and-add algorithm. Three inhouse physical phantoms containing tissue-surrogate materials were used to characterize the signal linearity, the signal difference-to-noise ratio (SDNR), the three-dimensional noise power spectrum (3D NPS), and the through-plane artifact spread function (ASF).While DPC-tomo highlights edges and interfaces in the image object, PC-tomo removes the differential nature of the DPC projection data and its pixel values are linearly related to the decrement of the real part of the x-ray refractive index. The SDNR values of polyoxymethylene in water and polystyrene in oil are 1.5 and 1.0, respectively, in AC-tomo, and the values were improved to 3.0 and 2.0, respectively, in PC-tomo. PC-tomo and AC-tomo demonstrate equivalent ASF, but their noise characteristics quantified by the 3D NPS were found to be different due to the difference in the tomosynthesis image reconstruction algorithms.It is feasible to simultaneously generate x-ray differential phase contrast, phase contrast, and absorption contrast tomosynthesis images using a grating-based data acquisition setup. The method shows promise in improving the visibility of several low-density materials and therefore merits further investigation.

Project description:BackgroundAccurate and complete response evaluation after treatment is important to implement individualized therapy for gastric cancer.PurposeTo investigate the effectiveness of diffusion kurtosis imaging (DKI) and in-line X-ray phase contrast imaging (ILXPCI) in the assessment of the therapeutic efficacy by transforming growth factor beta 1 (TGF-β1) inhibition.Study typeProspective animal study.Animal modelThirty nude mice subcutaneous xenotransplantation tumor model of gastric cancer for DKI and 10 peritoneal metastasis nude mice model for ILXPCI.Field strength/sequenceExaminations before and serially at 7, 14, 21, and 28 days after TGF-β1 inhibition treatment were performed at 3T MRI including T2 -weighted imaging (T2 WI) and DKI with five b values of 0, 500, 1000, 1500, 2000 s/mm2 ; ILXPCI examinations were performed at 14 days after treatment.AssessmentDKI parameters (apparent diffusion coefficient [ADC], diffusivity [D] and kurtosis [K]) were calculated by two experienced radiologists after postprocessing.Statistical testsFor the differences in all the parameters between the baseline and each timepoint for both the treated and the control mice, the Mann-Whitney test was used. The Spearman correlation test was used to evaluate correlations among the DKI parameters and corresponding pathologic necrosis fraction (NF).ResultsADC, D, and K values were significantly different between the two groups after treatment (P < 0.05). Serial measurements in the treated group showed that the ADC, D, and K values were significantly different at 7, 14, 21, and 28 days compared with baseline (P < 0.05). There were significant correlations between DKI parameters and NF (ADC, r = 0.865, P < 0.001; D, r = 0.802, P < 0.001; K, r = -0.944, P < 0.001). The ILXPCI results in the treated group showed a stronger absorption area than the control group.Data conclusionDKI may be used to evaluate the complete course therapeutic effects of gastric cancer induced by TGF-β1 inhibition, and the ILXPCI technique will improve the tumor microstructure resolution.Level of evidence1 Technical Efficacy: Stage 4 J. Magn. Reson. Imaging 2019;49:1553-1564.

Dataset Information

CO?-based in-line phase contrast imaging of small intestine in mice.

Publications

CO₂-based in-line phase contrast imaging of small intestine in mice.

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