Ontology highlight
ABSTRACT:
SUBMITTER: Li Y
PROVIDER: S-EPMC3727383 | biostudies-literature | 2011 Apr
REPOSITORIES: biostudies-literature
Li Yijun Y Cleary Ryan R Kellogg Mark M Soul Janet S JS Berry Gerard T GT Jensen Frances E FE
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 20110219 13-14
We have developed and validated a simple and sensitive stable isotope dilution liquid chromatography/tandem mass spectrometric (LC-MS/MS) method for the quantification of bumetanide in human serum. Samples were prepared with a simple acetonitrile based protein precipitation. The supernatant was then analyzed directly using LC-MS/MS. Chromatographic separation was achieved on a C18 reversed phase column using a methanol and water gradient. The detection was performed in selected reaction monitori ...[more]