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Preliminary crystallographic analysis of neuraminidase N2 from a new influenza A virus.

ABSTRACT: Influenza virus is a major viral respiratory pathogen that causes yearly epidemics in temperate climates. The H3N2 subtype is one of the major causative agents of severe epidemics and plays a critical role in vaccine development. The neuraminidase (NA) inhibitors oseltamivir and zanamivir are two commercially available NA-targeted competitive antiviral drugs. However, their effectiveness has been compromised by the rapid emergence of resistance. Q136K is a novel mutation in NA which confers resistance to zanamivir. In this study, a Q136K mutant N2 protein was expressed in a baculovirus system and crystals were obtained. The crystal of N2 belonged to space group P2?2?2?, with unit-cell parameters a = 109.5, b = 112.8, c = 165.2 Å. Data were collected to 2.4 Å resolution. Four monomers were found in the asymmetric unit. The Matthews coefficient and solvent content were calculated to be 3.0 Å³ Da?¹ and 59.0%, respectively.

SUBMITTER: Gao F

PROVIDER: S-EPMC3729159 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature

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Preliminary crystallographic analysis of neuraminidase N2 from a new influenza A virus.

Gao Feng F Bao Jinku J

Acta crystallographica. Section F, Structural biology and crystallization communications 20130727 Pt 8

Influenza virus is a major viral respiratory pathogen that causes yearly epidemics in temperate climates. The H3N2 subtype is one of the major causative agents of severe epidemics and plays a critical role in vaccine development. The neuraminidase (NA) inhibitors oseltamivir and zanamivir are two commercially available NA-targeted competitive antiviral drugs. However, their effectiveness has been compromised by the rapid emergence of resistance. Q136K is a novel mutation in NA which confers resi ...[more]

PMID: 23908028

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