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Expression, purification, crystallization and preliminary X-ray analysis of the extracellular sensory domain of DraK histidine kinase from Streptomyces coelicolor.

ABSTRACT: The bacterium Streptomyces coelicolor produces useful antibiotics from its secondary metabolites. DraK is a sensory histidine kinase involved in the differential regulation of antibiotics in S. coelicolor through the DraR/DraK two-component system. Here, the extracellular sensory domain of DraK was overexpressed in Escherichia coli, purified and crystallized using the sitting-drop vapour-diffusion method. The crystal diffracted to 2.2 Å resolution and belonged to space group C222?, with unit-cell parameters a = 41.91, b = 174.50, c = 145.25 Å, ? = ? = ? = 90°.

SUBMITTER: Yeo KJ

PROVIDER: S-EPMC3729172 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature

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Expression, purification, crystallization and preliminary X-ray analysis of the extracellular sensory domain of DraK histidine kinase from Streptomyces coelicolor.

Yeo Kwon Joo KJ Han Young-Hyun YH Eo Yumi Y Cheong Hae-Kap HK

Acta crystallographica. Section F, Structural biology and crystallization communications 20130727 Pt 8

The bacterium Streptomyces coelicolor produces useful antibiotics from its secondary metabolites. DraK is a sensory histidine kinase involved in the differential regulation of antibiotics in S. coelicolor through the DraR/DraK two-component system. Here, the extracellular sensory domain of DraK was overexpressed in Escherichia coli, purified and crystallized using the sitting-drop vapour-diffusion method. The crystal diffracted to 2.2 Å resolution and belonged to space group C222₁, with unit-cel ...[more]

PMID: 23908041

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