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Purification, crystallization and preliminary X-ray analysis of glucokinase from Streptomyces griseus in complex with glucose.

ABSTRACT: Glucokinase catalyzes the phosphorylation of glucose using ATP to yield glucose 6-phosphate. SgGlkA is a bacterial group III glucokinase from Streptomyces griseus that seems to play a regulatory role in carbon catabolite repression in this organism. SgGlkA was expressed in Escherichia coli, purified and crystallized using the sitting-drop vapour-diffusion method at 293?K. A crystal of SgGlkA in complex with glucose was obtained using a reservoir solution consisting of 0.9?M sodium/potassium tartrate, 0.2?M NaCl and 0.1?M imidazole pH 8.1 and diffracted X-rays to 1.84?Å resolution. The crystal of SgGlkA in complex with glucose belonged to space group P6(2)22 or P6(4)22, with unit-cell parameters a = b = 109.19, c = 141.18?Å. The crystal contained one molecule in the asymmetric unit.

SUBMITTER: Miyazono K

PROVIDER: S-EPMC3151127 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature

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Purification, crystallization and preliminary X-ray analysis of glucokinase from Streptomyces griseus in complex with glucose.

Miyazono Ken-ichi K Tabei Nobumitsu N Marushima Kazuya K Ohnishi Yasuo Y Horinouchi Sueharu S Tanokura Masaru M

Acta crystallographica. Section F, Structural biology and crystallization communications 20110720 Pt 8

Glucokinase catalyzes the phosphorylation of glucose using ATP to yield glucose 6-phosphate. SgGlkA is a bacterial group III glucokinase from Streptomyces griseus that seems to play a regulatory role in carbon catabolite repression in this organism. SgGlkA was expressed in Escherichia coli, purified and crystallized using the sitting-drop vapour-diffusion method at 293 K. A crystal of SgGlkA in complex with glucose was obtained using a reservoir solution consisting of 0.9 M sodium/potassium tart ...[more]

PMID: 21821894

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