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Preliminary X-ray crystallographic studies of the short form of the human TRAF4 TRAF domain.

ABSTRACT: TNF (tumour necrosis factor) receptor-associated factor 4 (TRAF4) is a unique TRAF protein that participates in morphogenetic and developmental function and cell migration. TRAF-family proteins contain a TRAF domain for target interaction. In this study, the short form of the human TRAF4 TRAF domain, corresponding to amino acids 290-462, was overexpressed in Escherichia coli using engineered C-terminal His tags. The short form of the TRAF4 TRAF domain was purified to homogeneity and crystallized at 293 K. Finally, X-ray diffraction data were collected to a resolution of 4.2 Å from a crystal belonging to the hexagonal space group P3?, with unit-cell parameters a = b = 147.17, c = 202.69 Å.

SUBMITTER: Yoon JH

PROVIDER: S-EPMC3729174 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature

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Preliminary X-ray crystallographic studies of the short form of the human TRAF4 TRAF domain.

Yoon Jong Hwan JH Park Hyun Ho HH

Acta crystallographica. Section F, Structural biology and crystallization communications 20130727 Pt 8

TNF (tumour necrosis factor) receptor-associated factor 4 (TRAF4) is a unique TRAF protein that participates in morphogenetic and developmental function and cell migration. TRAF-family proteins contain a TRAF domain for target interaction. In this study, the short form of the human TRAF4 TRAF domain, corresponding to amino acids 290-462, was overexpressed in Escherichia coli using engineered C-terminal His tags. The short form of the TRAF4 TRAF domain was purified to homogeneity and crystallized ...[more]

PMID: 23908043

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