Ontology highlight
ABSTRACT:
SUBMITTER: Xiao YL
PROVIDER: S-EPMC3731037 | biostudies-literature | 2013 Mar
REPOSITORIES: biostudies-literature
Xiao Yong-Li YL Kash John C JC Beres Stephen B SB Sheng Zong-Mei ZM Musser James M JM Taubenberger Jeffery K JK
The Journal of pathology 20130301 4
Most biopsy and autopsy tissues are formalin-fixed and paraffin-embedded (FFPE), but this process leads to RNA degradation that limits gene expression analysis. The RNA genome of the 1918 pandemic influenza virus was previously determined in a 9-year effort by overlapping RT-PCR from post-mortem samples. Here, the full genome of the 1918 virus at 3000× coverage was determined in one high-throughput sequencing run of a library derived from total RNA of a 1918 FFPE sample after duplex-specific nuc ...[more]