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Unusual base pairing during the decoding of a stop codon by the ribosome.

ABSTRACT: During normal translation, the binding of a release factor to one of the three stop codons (UGA, UAA or UAG) results in the termination of protein synthesis. However, modification of the initial uridine to a pseudouridine (?) allows efficient recognition and read-through of these stop codons by a transfer RNA (tRNA), although it requires the formation of two normally forbidden purine-purine base pairs. Here we determined the crystal structure at 3.1?Å resolution of the 30S ribosomal subunit in complex with the anticodon stem loop of tRNA(Ser) bound to the ?AG stop codon in the A site. The ?A base pair at the first position is accompanied by the formation of purine-purine base pairs at the second and third positions of the codon, which show an unusual Watson-Crick/Hoogsteen geometry. The structure shows a previously unsuspected ability of the ribosomal decoding centre to accommodate non-canonical base pairs.

SUBMITTER: Fernandez IS

PROVIDER: S-EPMC3732562 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature

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Unusual base pairing during the decoding of a stop codon by the ribosome.

Fernández Israel S IS Ng Chyan Leong CL Kelley Ann C AC Wu Guowei G Yu Yi-Tao YT Ramakrishnan V V

Nature 20130630 7460

During normal translation, the binding of a release factor to one of the three stop codons (UGA, UAA or UAG) results in the termination of protein synthesis. However, modification of the initial uridine to a pseudouridine (Ψ) allows efficient recognition and read-through of these stop codons by a transfer RNA (tRNA), although it requires the formation of two normally forbidden purine-purine base pairs. Here we determined the crystal structure at 3.1 Å resolution of the 30S ribosomal subunit in c ...[more]

PMID: 23812587

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