Ontology highlight
ABSTRACT:
SUBMITTER: Hsieh AC
PROVIDER: S-EPMC373385 | biostudies-literature | 2004
REPOSITORIES: biostudies-literature
Hsieh Andrew C AC Bo Ronghai R Manola Judith J Vazquez Francisca F Bare Olivia O Khvorova Anastasia A Scaringe Stephen S Sellers William R WR
Nucleic acids research 20040209 3
Gene silencing through RNA interference (RNAi) has been established as a means of conducting reverse genetic studies. In order to better understand the determinants of short interfering RNA (siRNA) knockdown for use in high-throughput cell-based screens, 148 siRNA duplexes targeting 30 genes within the PI3K pathway were selected and synthesized. The extent of RNA knockdown was measured for 22 genes by quantitative real-time PCR. Analysis of the parameters correlating with effective knockdown sho ...[more]