Ontology highlight
ABSTRACT:
SUBMITTER: Malek-Adamian E
PROVIDER: S-EPMC6686699 | biostudies-literature | 2019 Aug
REPOSITORIES: biostudies-literature
Malek-Adamian Elise E Fakhoury Johans J Arnold Amy E AE Martínez-Montero Saúl S Shoichet Molly S MS Damha Masad J MJ
Nucleic acid therapeutics 20190514 4
In this study, we explore the effect of a library of 2'-, 4'-, and 2',4'-modified uridine nucleosides and their impact on silencing firefly luciferase and on down-regulated in renal cell carcinoma (DRR) gene targets. The modifications studied were 2'-F-ribose, 2'-F-arabinose, 2'-OMe-ribose, 2'-F,4'-OMe-ribose, 2'-F,4'-OMe-arabinose, and 2'-OMe,4'-F-ribose. We found that 2',4'-modifications are well tolerated within A-form RNA duplexes, leading to virtually no change in melting temperature as ass ...[more]