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Estimation of glomerular podocyte number: a selection of valid methods.

ABSTRACT: The podocyte depletion hypothesis has emerged as an important unifying concept in glomerular pathology. The estimation of podocyte number is therefore often a critical component of studies of progressive renal diseases. Despite this, there is little uniformity in the biomedical literature with regard to the methods used to estimate this important parameter. Here we review a selection of valid methods for estimating podocyte number: exhaustive enumeration method, Weibel and Gomez method, disector/Cavalieri combination, disector/fractionator combination, and thick-and-thin section method. We propose the use of the disector/fractionator method for studies in which controlled sectioning of tissue is feasible, reserving the Weibel and Gomez method for studies based on archival or routine pathology material.

SUBMITTER: Lemley KV

PROVIDER: S-EPMC3736716 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature

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Estimation of glomerular podocyte number: a selection of valid methods.

Lemley Kevin V KV Bertram John F JF Nicholas Susanne B SB White Kathryn K

Journal of the American Society of Nephrology : JASN 20130705 8

The podocyte depletion hypothesis has emerged as an important unifying concept in glomerular pathology. The estimation of podocyte number is therefore often a critical component of studies of progressive renal diseases. Despite this, there is little uniformity in the biomedical literature with regard to the methods used to estimate this important parameter. Here we review a selection of valid methods for estimating podocyte number: exhaustive enumeration method, Weibel and Gomez method, disector ...[more]

PMID: 23833256

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