Unknown

Dataset Information

0

Binding, conformational transition and dimerization of amyloid-? peptide on GM1-containing ternary membrane: insights from molecular dynamics simulation.


ABSTRACT: Interactions of amyloid-? (A?) with neuronal membrane are associated with the progression of Alzheimer's disease (AD). Ganglioside GM1 has been shown to promote the structural conversion of A? and increase the rate of peptide aggregation; but the exact nature of interaction driving theses processes remains to be explored. In this work, we have carried out atomistic-scale computer simulations (totaling 2.65 µs) to investigate the behavior of A? monomer and dimers in GM1-containing raft-like membrane. The oligosaccharide head-group of GM1 was observed to act as scaffold for A?-binding through sugar-specific interactions. Starting from the initial helical peptide conformation, a ?-hairpin motif was formed at the C-terminus of the GM1-bound A?-monomer; that didn't appear in absence of GM1 (both in fluid POPC and liquid-ordered cholesterol/POPC bilayers and also in aqueous medium) within the simulation time span. For A?-dimers, the ?-structure was further enhanced by peptide-peptide interactions, which might influence the propensity of A? to aggregate into higher-ordered structures. The salt-bridges and inter-peptide hydrogen bonds were found to account for dimer stability. We observed spontaneous formation of intra-peptide D(23)-K(28) salt-bridge and a turn at V(24)GSN(27) region - long been accepted as characteristic structural-motifs for amyloid self-assembly. Altogether, our results provide atomistic details of A?-GM1 and A?-A? interactions and demonstrate their importance in the early-stages of GM1-mediated A?-oligomerisation on membrane surface.

SUBMITTER: Manna M 

PROVIDER: S-EPMC3739818 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

altmetric image

Publications

Binding, conformational transition and dimerization of amyloid-β peptide on GM1-containing ternary membrane: insights from molecular dynamics simulation.

Manna Moutusi M   Mukhopadhyay Chaitali C  

PloS one 20130809 8


Interactions of amyloid-β (Aβ) with neuronal membrane are associated with the progression of Alzheimer's disease (AD). Ganglioside GM1 has been shown to promote the structural conversion of Aβ and increase the rate of peptide aggregation; but the exact nature of interaction driving theses processes remains to be explored. In this work, we have carried out atomistic-scale computer simulations (totaling 2.65 µs) to investigate the behavior of Aβ monomer and dimers in GM1-containing raft-like membr  ...[more]

Similar Datasets

| S-EPMC556260 | biostudies-literature
| S-EPMC6497634 | biostudies-literature
| S-EPMC8153327 | biostudies-literature
| S-EPMC2599820 | biostudies-literature
| S-EPMC5089616 | biostudies-literature
| S-EPMC8173568 | biostudies-literature
| S-EPMC2924059 | biostudies-literature
| S-EPMC3963130 | biostudies-literature
| S-EPMC10259223 | biostudies-literature
| S-EPMC10872638 | biostudies-literature