Conformational transition of membrane-associated terminally acylated HIV-1 Nef.
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ABSTRACT:
SUBMITTER: Akgun B
PROVIDER: S-EPMC4011395 | biostudies-literature | 2013 Oct
REPOSITORIES: biostudies-literature
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Conformational transition of membrane-associated terminally acylated HIV-1 Nef.
Akgun Bulent B Satija Sushil S Nanda Hirsh H Pirrone Gregory F GF Shi Xiaomeng X Engen John R JR Kent Michael S MS
Structure (London, England : 1993) 20130912 10
Many proteins are posttranslationally modified by acylation targeting them to lipid membranes. While methods such as X-ray crystallography and nuclear magnetic resonance are available to determine the structure of folded proteins in solution, the precise position of folded domains relative to a membrane remains largely unknown. We used neutron and X-ray reflection methods to measure the displacement of the core domain of HIV Nef from lipid membranes upon insertion of the N-terminal myristate gro ...[more]
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