A new method for stranded whole transcriptome RNA-seq.
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ABSTRACT:
SUBMITTER: Miller DF
PROVIDER: S-EPMC3739992 | biostudies-literature | 2013 Sep
REPOSITORIES: biostudies-literature
Publications
A new method for stranded whole transcriptome RNA-seq.
Miller David F B DF Yan Pearlly S PS Buechlein Aaron A Rodriguez Benjamin A BA Yilmaz Ayse S AS Goel Shokhi S Lin Hai H Collins-Burow Bridgette B Rhodes Lyndsay V LV Braun Chris C Pradeep Sunila S Rupaimoole Rajesha R Dalkilic Mehmet M Sood Anil K AK Burow Matthew E ME Tang Haixu H Huang Tim H TH Liu Yunlong Y Rusch Douglas B DB Nephew Kenneth P KP
Methods (San Diego, Calif.) 20130401 2
This report describes an improved protocol to generate stranded, barcoded RNA-seq libraries to capture the whole transcriptome. By optimizing the use of duplex specific nuclease (DSN) to remove ribosomal RNA reads from stranded barcoded libraries, we demonstrate improved efficiency of multiplexed next generation sequencing (NGS). This approach detects expression profiles of all RNA types, including miRNA (microRNA), piRNA (Piwi-interacting RNA), snoRNA (small nucleolar RNA), lincRNA (long non-co ...[more]
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