Ontology highlight
ABSTRACT:
SUBMITTER: Harrington CA
PROVIDER: S-EPMC7156519 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Harrington Christina A CA Fei Suzanne S SS Minnier Jessica J Carbone Lucia L Searles Robert R Davis Brett A BA Ogle Kimberly K Planck Stephen R SR Rosenbaum James T JT Choi Dongseok D
Scientific reports 20200414 1
Peripheral blood is a highly accessible biofluid providing a rich source of information about human physiology and health status. However, for studies of the blood transcriptome with RNA sequencing (RNA-Seq) techniques, high levels of hemoglobin mRNAs (hgbRNA) present in blood can occupy valuable sequencing space, impacting detection and quantification of non-hgbRNAs. In this study, we evaluated two methods for preparing ribosomal RNA (rRNA)-depleted sequencing libraries for RNA-Seq of whole blo ...[more]