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Acute mechanical stretch promotes eNOS activation in venous endothelial cells mainly via PKA and Akt pathways.

ABSTRACT: In the vasculature, physiological levels of nitric oxide (NO) protect against various stressors, including mechanical stretch. While endothelial NO production in response to various stimuli has been studied extensively, the precise mechanism underlying stretch-induced NO production in venous endothelial cells remains incompletely understood. Using a model of continuous cellular stretch, we found that stretch promoted phosphorylation of endothelial NO synthase (eNOS) at Ser¹¹??, Ser?³³ and Ser?¹? and NO production in human umbilical vein endothelial cells. Although stretch activated the kinases AMPK?, PKA, Akt, and ERK1/2, stretch-induced eNOS activation was only inhibited by kinase-specific inhibitors of PKA and PI3K/Akt, but not of AMPK? and Erk1/2. Similar results were obtained with knockdown by shRNAs targeting the PKA and Akt genes. Furthermore, inhibition of PKA preferentially attenuated eNOS activation in the early phase, while inhibition of the PI3K/Akt pathway reduced eNOS activation in the late phase, suggesting that the PKA and PI3K/Akt pathways play distinct roles in a time-dependent manner. Finally, we investigated the role of these pathways in stretch-induced endothelial exocytosis and leukocyte adhesion. Interestingly, we found that inhibition of the PI3K/Akt pathway increased stretch-induced Weibel-Palade body exocytosis and leukocyte adhesion, while inhibition of the PKA pathway had the opposite effects, suggesting that the exocytosis-promoting effect of PKA overwhelms the inhibitory effect of PKA-mediated NO production. Taken together, the results suggest that PKA and Akt are important regulators of eNOS activation in venous endothelial cells under mechanical stretch, while playing different roles in the regulation of stretch-induced endothelial exocytosis and leukocyte adhesion.

SUBMITTER: Hu Z

PROVIDER: S-EPMC3743752 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Acute mechanical stretch promotes eNOS activation in venous endothelial cells mainly via PKA and Akt pathways.

Hu Zhenqian Z Xiong Yan Y Han Xiaofan X Geng Chenyang C Jiang Beibei B Huo Yingqing Y Luo Jincai J

PloS one 20130814 8

In the vasculature, physiological levels of nitric oxide (NO) protect against various stressors, including mechanical stretch. While endothelial NO production in response to various stimuli has been studied extensively, the precise mechanism underlying stretch-induced NO production in venous endothelial cells remains incompletely understood. Using a model of continuous cellular stretch, we found that stretch promoted phosphorylation of endothelial NO synthase (eNOS) at Ser¹¹⁷⁷, Ser⁶³³ and Ser⁶¹⁵ ...[more]

PMID: 23977025

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Dataset Information

Acute mechanical stretch promotes eNOS activation in venous endothelial cells mainly via PKA and Akt pathways.

Publications

Acute mechanical stretch promotes eNOS activation in venous endothelial cells mainly via PKA and Akt pathways.

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